LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(2135..2231) /label=attL2 /note="attL2 (c)" misc_signal complement(2246..2265) /note="T7 Promoter/priming site (c)" primer_bind 2273..2289 /note="M13 Reverse priming site" gene 2402..3211 /note="Kanamycin resistance gene" rep_origin 3332..4005 /note="pUC origin" vector join(2143..4008,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..2128 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:5302 IMAGE:3456869" /tissue_type="Cervix, carcinoma" /clone_lib="NIH_MGC_12" /lab_host="DH10B" /note="Vector: pCMV-SPORT6" gene 692..2128 /gene="KDELC1" /gene_synonym=EP58 /gene_synonym=KDEL1 /gene_synonym=MGC5302 /db_xref="GeneID:79070" /db_xref="HGNC:19350" CDS 692..2128 /dnas_title="KDEL (Lys-Asp-Glu-Leu) containing 1" /gene="KDELC1" /gene_synonym=EP58 /gene_synonym=KDEL1 /gene_synonym=MGC5302 /codon_start=1 /product="KDEL (Lys-Asp-Glu-Leu) containing 1" /protein_id="AAH01297.1" /db_xref="GI:12654903" /db_xref="GeneID:79070" /db_xref="HGNC:19350" /translation="MFGTLLLYCFFLATVPALAETGGERQLSPEKSEIWGPGLKADVV LPARYFYIQAVDTSGNKFTSSPGEKVFQVKVSAPEEQFTRVGVQVLDRKDGSFIVRYR MYASYKNLKVEVKFQGQHVAKSPYILKGPVYHENCDCPLQDSAAWLREMNCPETIAQI QRDLAHFPAVDPEKIAVEIPKRFGQRQSLCHYTLKDNKVYIKTHGEHVGFRIFMDAIL LSLTRKVKMPDVELFVNLGDWPLEKKKSNSNIHPIFSWCGSTDSKDIVMPTYDLTDSV LETMGRVSLDMMSVQANTGPPWESKNSTAVWRGRDSRKERLELVKLSRKHPELIDAAF TNFFFFKHDENLYGPIVKHISFFDFFKHKYQINIDGTVAAYRLPYLLVGDSVVLKQDS IYYEHFYNELQPWKHYIPVKSNLSDLLEKLKWAKDHDEEAKKIAKAGQEFARNNLMGD DIFCYYFKLFQEYANLQVSEPQIREGMKRVEPQTEDDLFPCTCHRKKTKDEL" misc_difference 974..974 /gene="KDELC1" /gene_synonym=EP58 /gene_synonym=KDEL1 /gene_synonym=MGC5302 /note="'G' in cDNA is 'A' in the human genome; amino acid difference: 'V' in cDNA, 'I' in the human genome. The chimpanzee genome agrees with the human genomic sequence and not the cDNA." misc_feature 692..2128 /note="KDELC1 coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgagaccggc ggagaaaggc agctgagccc 721 ggagaagagc gaaatatggg gacccgggct aaaagcagac gtcgtccttc ccgcccgcta 781 tttctatatt caggcagtgg atacatcagg gaataaattc acatcttctc caggcgaaaa 841 ggtcttccag gtgaaagtct cagcaccaga ggagcaattc actagagttg gagtccaggt 901 tttagaccga aaagatgggt ccttcatagt aagatacaga atgtatgcaa gctacaaaaa 961 tctgaaggtg gaagttaaat tccaagggca acatgtggcc aaatccccat atattttaaa 1021 agggccggtt taccatgaga actgtgactg tcctctgcaa gatagtgcag cctggctacg 1081 ggagatgaac tgccctgaaa ccattgctca gattcagaga gatctggcac atttccctgc 1141 tgtggatcca gaaaagattg cagtagaaat cccaaaaaga tttggacaga ggcagagcct 1201 atgtcactac accttaaagg ataacaaggt ttatatcaag actcatggtg aacatgtagg 1261 ttttagaatt ttcatggatg ccatactact ttctttgact agaaaggtga agatgccaga 1321 tgtggagctc tttgttaatt tgggagactg gcctttggaa aaaaagaaat ccaattcaaa 1381 catccatccg atcttttcct ggtgtggctc cacagattcc aaggatatcg tgatgcctac 1441 gtacgatttg actgattctg ttctggaaac catgggccgg gtaagtctgg atatgatgtc 1501 cgtgcaagct aacacgggtc ctccctggga aagcaaaaat tccactgccg tctggagagg 1561 gcgagacagc cgcaaagaga gactcgagct ggttaaactc agtagaaaac acccagaact 1621 catagacgct gctttcacca actttttctt ctttaaacac gatgaaaacc tgtatggtcc 1681 cattgtgaaa catatttcat tttttgattt cttcaagcat aagtatcaaa taaatatcga 1741 tggcactgta gcagcttatc gcctgccata tttgctagtt ggtgacagtg ttgtgctgaa 1801 gcaggattcc atctactatg aacattttta caatgagctg cagccctgga aacactacat 1861 tccagttaag agcaacctga gcgatctgct agaaaaactt aaatgggcga aagatcacga 1921 tgaagaggcc aaaaagatag caaaagcagg acaagaattt gcaagaaata atctcatggg 1981 cgatgacata ttctgttatt atttcaaact tttccaggaa tatgccaatt tacaagtgag 2041 tgagccccaa atccgagagg gcatgaaaag ggtagaacca cagactgagg acgacctctt 2101 cccttgtact tgccatagga aaaagaccTA GGACCCAGCT TTCTTGTACA AAGTTGGCAT 2161 TATAAGAAAG CATTGCTTAT CAATTTGTTG CAACGAACAG GTCACTATCA GTCAAAATAA 2221 AATCATTATT TGCCATCCAG CTGATATCCC CTATAGTGAG TCGTATTACA TGGTCATAGC 2281 TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA AAATCTCTGA TGTTACATTG CACAAGATAA 2341 AATAATATCA TCATGAACAA TAAAACTGTC TGCTTACATA AACAGTAATA CAAGGGGTGT 2401 TATGAGCCAT ATTCAACGGG AAACGTCGAG GCCGCGATTA AATTCCAACA TGGATGCTGA 2461 TTTATATGGG TATAAATGGG CTCGCGATAA TGTCGGGCAA TCAGGTGCGA CAATCTATCG 2521 CTTGTATGGG AAGCCCGATG CGCCAGAGTT GTTTCTGAAA CATGGCAAAG GTAGCGTTGC 2581 CAATGATGTT ACAGATGAGA TGGTCAGACT AAACTGGCTG ACGGAATTTA TGCCTCTTCC 2641 GACCATCAAG CATTTTATCC GTACTCCTGA TGATGCATGG TTACTCACCA CTGCGATCCC 2701 CGGAAAAACA GCATTCCAGG TATTAGAAGA ATATCCTGAT TCAGGTGAAA ATATTGTTGA 2761 TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA TTCGATTCCT GTTTGTAATT GTCCTTTTAA 2821 CAGCGATCGC GTATTTCGTC TCGCTCAGGC GCAATCACGA ATGAATAACG GTTTGGTTGA 2881 TGCGAGTGAT TTTGATGACG AGCGTAATGG CTGGCCTGTT GAACAAGTCT GGAAAGAAAT 2941 GCATAAACTT TTGCCATTCT CACCGGATTC AGTCGTCACT CATGGTGATT TCTCACTTGA 3001 TAACCTTATT TTTGACGAGG GGAAATTAAT AGGTTGTATT GATGTTGGAC GAGTCGGAAT 3061 CGCAGACCGA TACCAGGATC TTGCCATCCT ATGGAACTGC CTCGGTGAGT TTTCTCCTTC 3121 ATTACAGAAA CGGCTTTTTC AAAAATATGG TATTGATAAT CCTGATATGA ATAAATTGCA 3181 GTTTCATTTG ATGCTCGATG AGTTTTTCTA ATCAGAATTG GTTAATTGGT TGTAACACTG 3241 GCAGAGCATT ACGCTGACTT GACGGGACGG CGCAAGCTCA TGACCAAAAT CCCTTAACGT 3301 GAGTTACGCG TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG 3361 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC 3421 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 3481 CAGAGCGCAG ATACCAAATA CTGTTCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA 3541 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC 3601 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC 3661 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA 3721 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 3781 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT 3841 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA 3901 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC 3961 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTT //